Sequence differences between glycosylated and non-glycosylated Asn-X-Thr/Ser acceptor sites: implications for protein engineering
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چکیده
منابع مشابه
Calreticulin functions in vitro as a molecular chaperone for both glycosylated and non-glycosylated proteins.
Calreticulin (CRT) is thought to be a molecular chaperone that interacts with glycoproteins exclusively through a lectin site specific for monoglucosylated oligosaccharides. However, this chaperone function has never been directly demonstrated nor is it clear how lectin-oligosaccharide interactions facilitate glycoprotein folding. Using purified components, we show that CRT suppresses the aggre...
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Bone morphogenetic proteins (BMP), in particular BMP-2, are the growth factors primarily responsible for osteoinduction. A knowledge of interactions between bone substitute materials and growth factor variants is crucial to designing bone substitutes with an ideal release profile. Here we compare glycosylated and non-glycosylated recombinant human bone morphogenetic protein-2 (rhBMP-2) either i...
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ژورنال
عنوان ژورنال: "Protein Engineering, Design and Selection"
سال: 1990
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/3.5.433